PRO-003 Protein purification

This is the most versatile and most demanding step of the expression process. Entelechon has developed a number of protocols that give us plenty of options for the purification of virtually any protein. Starting from simple ion exchange chromatography via affinity chromatography up to complex strategies based on stacking several purification steps and using multiple affinity tags, our purification procedures are adjusted to the desired purity and nature of the target protein.
Using rigorous QC based on chromatography, SDS-PAGE, Western Blot, ELISA, and mass spectrometry, not only can we ensure the required level of purity, but prove it.
If a protein is required in its native, functional state, we leverage our expertise in reconstituting even delicate protein structures. We like to work closely with the client in developing suitable assays for functionality, often incorporating and adapting pre-existing tests developed by the client.
Available purification options are: