PRO-004 Protein characterization
Entelechon offers several services for protein characterization. Besides standard QC methods such as SDS PAGE, Western Blot and UV/Vis spectrometry, total amino acid analysis provides an accurate quantification of a pure protein, whereas N-terminal sequencing allows to quickly confirm the identity of a protein or peptide.
At least 10 pmol of pure protein/peptide with >80% purity are required, to be provided on PVDF membrane or in a low salt buffer solution, free of amines.
Sequencing will be performed stepwise, starting with the first five amino acids. We follow the Edman degradation and can sequence up to 10 amino acids.
If sequencing of the inital five amino acids is unsuccessful, a setup fee applies.
For the total amino acid analysis 1 – 5 μg of the protein, 0.2 – 1 μg of the peptide, respectively, are required.
Please inquire about the specifications for SDS PAGE, Western Blot and spectrometry.
| SDS PAGE | PRO-004-001 |
| Western Blot | PRO-004-002 |
| UV/Vis spectrometry | PRO-004-003 |
| N-terminal sequencing | PRO-004-004 |
| Total amino acid analysis | PRO-004-005 |
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