Online tool for the calculation of randomized mutant libraries

Entelechon has published a free online tool for the calculation of the complexity of randomized mutant libraries. This tool allows to enter the number of randomized amino acid positions, as well as the number of codon variants at each position, in order to estimate the total number of possible permutations. Based on this complexity, the tool tests whether a synthetic library will contain sufficient molecules in order to cover each variant at least once.

In addition, the tool compares the performance of codon-precision libraries with conventional libraries based on single nucleotide randomizations. This allows the user to choose the most economic and efficient approach for the problem at hand.

The tool can be found here.