Saito H, Fujita Y, Kashida S, Hayashi K, Inoue T.
Synthetic human cell fate regulation by protein-driven RNA switches
Nature Communications 2011 Jan;2(1):160.
[1] Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. [2] International Cooperative Research Project, Japan Science and Technology Agency, 5 Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan. [3] The Hakubi Center, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
Short summary: The paper describes a new method in synthetic biology using an intracellular protein as a trigger for regulating human cell fate. The authors suppose that the method has potential for curing cellular defects or improving the intracellular production of useful molecules by bypassing or rewiring intrinsic signal networks.
Fisher MA, McKinley KL, Bradley LH, Viola SR, Hecht MH.
De novo designed proteins from a library of artificial sequences function in Escherichia coli and enable cell growth.
PLoS One. 2011 Jan 4;6(1):e15364.
Departments of Chemistry and Molecular Biology, Princeton University, Princeton, New Jersey, United States of America.
Short summary: The paper describes the first use of artificial gene products synthesized to replace natural proteins and to maintain the growth of living cells. A collection of more than 106 de novo designed proteins were tested for the ability to provide biological functions to sustain cell growth in different knockout strains of E. coli. Four different strains were rescued by the artificial gene products. This paper is regarded as a major breakthrough in the emerging field of synthetic biology.
Ellis T, Adie T, Baldwin GS.
DNA assembly for synthetic biology: from parts to pathways and beyond.
Integr Biol (Camb). 2011 Jan 19
Centre for Synthetic Biology and Innovation, South Kensington, Imperial College London, SW7 2AZ, UK.
Short summary: The paper reviews the different techniques used to assembly synthetic DNA and gives an outlook how this important aspect of synthetic biology may proceed in the future.