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Presidential Commission for the Study of Bioethical Issues

The Presidential Commission for the Study of Bioethical Issues has submitted their synthetic biology report to President Obama in December. Since then it was often disscussed in public, last time on 3rd of February in the Center for American Progress. Next meeting of the Comission is in Washington, D.C.on Feb. 28 – March 1, 2011.

For more information visit: http://www.bioethics.gov/

70th Harden conference: ”Synthetic biology: design and engineering through understanding”

As part of the 70th Harden conferences the biochemical society UK will organize a conference about synthetic biology. The conference” Synthetic biology: design and engineering through understanding” will take place from August 22-26 at the Keele University (UK) and should illustrate the potential of this emerging cross-disciplinary area.
Early application and abstract deadline: 22 June 2011.

Website: http://www.biochemistry.org/tabid/379/MeetingNo/70HDN/view/Conference/default.aspx

iGEM goes regional

The competition for synthetic biology, iGEM, will be organized this year for the eighth time. This year the organizers from the MIT decided to reorganize the competition and to organize Regional Jamborees in Asia, Europe and America. The winning teams of the regional competitions will advance to the World Championship Jamboree at MIT. The changes have become necessary as the number of teams in iGEM has continued to increase (number of participants in 2010: 1300).

Source: http://2010.igem.org/Main_Page

Highlighted new synthetic biology publications 2011

Saito H, Fujita Y, Kashida S, Hayashi K, Inoue T.

Synthetic human cell fate regulation by protein-driven RNA switches

Nature Communications 2011 Jan;2(1):160.

[1] Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. [2] International Cooperative Research Project, Japan Science and Technology Agency, 5 Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan. [3] The Hakubi Center, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.

Short summary: The paper describes a new method in synthetic biology using an intracellular protein as a trigger for regulating human cell fate. The authors suppose that the method has potential for curing cellular defects or improving the intracellular production of useful molecules by bypassing or rewiring intrinsic signal networks.

Fisher MA, McKinley KL, Bradley LH, Viola SR, Hecht MH.

De novo designed proteins from a library of artificial sequences function in Escherichia coli and enable cell growth.

PLoS One. 2011 Jan 4;6(1):e15364.

Departments of Chemistry and Molecular Biology, Princeton University, Princeton, New Jersey, United States of America.

Short summary: The paper describes the first use of artificial gene products synthesized to replace natural proteins and to maintain the growth of living cells. A collection of more than 106 de novo designed proteins were tested for the ability to provide biological functions to sustain cell growth in different knockout strains of E. coli. Four different strains were rescued by the artificial gene products. This paper is regarded as a major breakthrough in the emerging field of synthetic biology.

Ellis T, Adie T, Baldwin GS.

DNA assembly for synthetic biology: from parts to pathways and beyond.

Integr Biol (Camb). 2011 Jan 19

Centre for Synthetic Biology and Innovation, South Kensington, Imperial College London, SW7 2AZ, UK.

Short summary: The paper reviews the different techniques used to assembly synthetic DNA and gives an outlook how this important aspect of synthetic biology may proceed in the future.

Happy Mole day

We wish all fellow researchers and biologists a wonderful and happy Mole day. Today being October 23, it is a great opportunity to commemorate this unit so essential to our work. So let’s hear it for the Mole, even if the day is not yet an official holiday. But be careful: Mole day is only between 6.02 AM and 6.02 PM – so no unduly celebration in the early morning or evening, lest you want to walk the plank into a pool of 6.02 x 1023 molecules of water…

Site-directed mutagenesis

You want to modify an existing DNA template? For instance, exchange an amino acid, add a restriction site or disrupt undesirable DNA motifs?

We can perform a site-directed mutagenesis according to your specification and requirements. If required, we can help with the design of the target sequence.

You will receive the mutagenized fragment along with a detailed report on the mutagenesis and an extended QC. Quality control will include the complete sequencing of the resulting DNA fragment.

Starting at EUR 299.00, this service can help you to exploit the full potential of your existing DNA fragments.

Send your inquiry via our secure online form

Synthesis of PCR controls

You are working on an (RT-) PCR assay and need a specific and unique control?

We can design control fragments with very specific physical and sequence properties. Taking into consideration secondary structure formation, melting temperature and melting behaviour, unique non-homologous sequence and restriction sites, we will design a control template that is reliable, tested for performance and convenient to use.

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Transcript detection and identification

You want to investigate related transcripts of the same gene? We can design specific PCR assays for the detection and quantification of individual transcripts.

Based on our proprietary software for primer and oligonucleotide probe design, we will analyze the gene or gene family at question and come up with a set of specific PCR primers which detect individual transcripts.

We will be happy to advise you on the optimal detection strategy and primer design.

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Primer design for quantitative PCR

You want to perform quantitative PCR on one or more target genes? Drawing from our expertise on genomics analysis, primer and probe design, we can design PCR primers and DNA probes for you.

Using advanced proprietary design software, we can adapt to your requirements with regard to physical properties and specificity.

We will take into consideration primer melting temperature, secondary structure formation, cross-hybridization and gene family relationships.

We can help you creating specific, sensitive assays for the detection of DNA and RNA target genes in virtually any setting.

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Small scale expression screening

Need to test the expression level of a gene? We can do that for you. Our so-called best clone screen tests a set of E. coli clones for expression on a 1ml scale.

The screen will include a range of expression conditions defined by you, and a test of solubility. Optionally, an affinity chromatography can be added.

Starting at EUR 990.00, this service comes with a full report on the screening results, as well as suggestions for improved expression yields.

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