Leto 1.0 is our proprietary software for the optimization of DNA sequences for maximum expression yields. It features a new approach towards gene optimization, based on a genetic algorithm. This approach allows for the simultaneous optimization of a large set of competing parameters, such as

• Codon usage
• Homogenous GC content
• mRNA secondary structure
• Cryptic splice sites
• Codon and motif repeats
• Restriction sites
• Custom motives (e.g. Poly-A signals, GC islets)

Leto is capable of searching the vast solution space of possible sequence permutations and find a sequence optimized for all of these (and more) parameters.

In addition, Leto offers a convenient graphical user interface for working with DNA sequences. For example, restriction sites can be inserted with a few mouse clicks, keeping the amino acid sequence unchanged.

System requirements

Operating system: Windows XP, Windows 2000, Windows 98, Mac OS X, Linux, Solaris
CPU: Pentium 3.00 GHz or higher, or equivalent processor
RAM: 1024 MBytes or more
Hard disk: 20 MBytes or more

Open source libraries

Leto makes use of some open source libraries. Follow this link for details on and links to these libraries.

Contact

If you have questions regarding Leto 1.0, or gene optimization in general, please contact Mr. Markus Fischer at tel. +49 9405 96999 15 and at fischer@entelechon.com.

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