Directed evolution
Entelechon can assist your directed evolution projects by a rich combination of bioinformatics and DNA library synthesis.
Our track record includes the design and execution of over 100 gene library projects as well as participation in protein engineering projects that aimed at increasing the efficiency or specificity of enzymatic functions.
Gene library design
When designing randomized gene libraries, great care must be taken to control the number of possible permutations and to ensure coverage of all permutations. Our in-depth analysis will ensure both, and will lead to a design that is both cost-efficient and reliable.
We offer codon-precision randomized libraries, where a precisely defined set of codons is incorporated at a specific position. This avoids undesired variants and stop codons of conventional random libraries.
Quality parameters
Our expertise entails the design of randomized gene libraries as well as the synthesis with high fidelity. The resulting libraries have a mismatch incorporation rate typically below 0.1%.
The distribution of randomized nucleotides matches the desired distribution very closely, usually with a deviation below 10%.
How to start
We are happy to discuss your directed evolution project with you. Please contact us by email or telephone for an initial project analysis. Our experts will work closely with you in defining requirements and providing a detailed price quote and project proposal.