perfectGene is Entelechon’s line of custom gene synthesis services. Being one of the first companies fully focused on gene synthesis, Entelechon has created a robust platform for the assembly of virtually any gene sequence.

Please use our online gene synthesis order form to submit your gene order/request.

Description of the perfectGene service

Based on a gene or protein sequence, which we will either use as-is or optimize for maximum gene expression, a gene is assembled from chemically synthesized oligonucleotides. The gene is then cloned into a standard vector, and a 100% correct clone is picked by sequence screening. The deliverables include:

• 1-5 µg of purified standard vector containing the synthesized gene
• Sequence chromatograms that completely cover the synthesized gene
• 100% guaranteed sequence accuracy
• Synthesis report, including a vector map

The synthesis process can be tracked via a web interface that provides detailed information on the status of a gene as well as a secure way of transferring confidential data between Entelechon and the client.

Orders and inquiries

Please refer to the order form for submitting an order. The form uses state-of-the-art data encryption for the transfer of sequence data to Entelechon. For price inquiries, please use this form.

Gene order form for Israel customers

For customers from Israel, we would like to make you aware of our partnership with our Israeli distributor Syntezza. Please use the Syntezza order form.

Requirements

In order to start a gene synthesis, we just need a DNA or protein sequence of the target gene. In those cases where sequence data is not available, our bioinformatics unit can provide a search and retrieval service which identifies the sequence based on an accession number, gene name, or other criteria. We can also design consensus sequences from a set of homologous genes.

Ligation product and randomized libraries

Most genes are shipped cloned into a vector. This ensures that we can select a 100% correct clone. However, in some instances customers require a free double-strand instead. In these cases, we can ship the so-called ligation product, which is the immediate result of assembling the oligos into the full-length gene. The ligation product will inevitably contain a certain degree of erroneous DNA strands, and thus needs further screening when cloned into a vector.

However, the advantage of the ligation product is that it can contain randomized nucleotide positions where two, three or all four nucleotides are randomly incorporated at a given location within the sequence. If you are interested in library construction, please send an inquiry via the contact form. We have designed dedicated synthesis protocols for optimal library quality.

It is important to bear in mind that the overall quality of DNA libraries is limited by physical constraints. Since oligonucleotide chemistry has a limited fidelity of about 99.5% per coupling, libraries will contain mismatch mutations and deletions. Our process ensures selection of correct sequences, and therefore our error rate is usually in the range of 0.1% per nucleotide, depending on the length of the sequence and the number and kind of randomized nucleotides.

Still, at this error rate, for a gene of 800bp length, about 45% of DNA double strands will contain at least one undesired error.

For an in-depth discussion of libraries, see also our technical bulletin.

Vector construction

We leverage our gene assembly technology for the efficient de novo construction of vectors according to your specifications. Entelechon’s bioinformatics will assist in the design of a vector, and QC will ensure that the client receives a fully characterized and 100% correct plasmid.

Confidentiality

We ensure the highest possible level of confidentiality for customer data, in particular DNA and protein sequences. Please ask for a confidentiality agreement, when ordering or inquirying for custom gene synthesis.

All sequences are treated strictly confidential, and we have set up physical and digital barriers to ensure the safety of your data. Our customer web interface uses a 128bit SSL encrypted connection for maximum data security.

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