Gene to Protein All Inclusive Service

Starting with a sequence file, we provide a readily purified protein produced in HEK293 or CHO cells.

We will produce purified protein from 1 litre of HEK293 or CHO cells*, including the complete workflow:

Gene optimization with Leto

Leto 1.0 is our proprietary software for the optimization of DNA sequences for maximum expression yields. Leto features a new approach towards gene optimization, based on a genetic algorithm.

Gene synthesis (up to 3 kbp)

Entelechon was one of the first companies fully focused on gene synthesis. Based on broad experience, Entelechon has created a robust platform for the assembly of virtually any gene sequence.

Sequencing

Cloning

Subcloning of the synthesised gene into our in-house expression vectors is included in the package. Entelechon offers also subcloning of the gene into a custom expression vector; for this additional service an extra fee applies.

Expression in HEK293 or CHO cells (1 litre)

Expression of target protein in 1 litre of transiently transfected HEK293 or CHO cells. Mammalian expression systems are more suitable than lower eukaryotes or prokaryotes for providing soluble proteins that possess relevant post-translational modifications.

Purification of protein

The target protein, secreted in the cell culture medium, will be purified via his tag or protein A column. A second purification step like SEC or IEC can be booked separately.

Analysis and Documentation

The package includes protein analysis by SDS-PAGE and Western Blot and documentation of the purification process.

Turn around time: Typically 6-8 weeks

Package price: EUR 4950.00

Please click here for the order form

* The yield of the expression may vary and depends on the specific expression level of the gene, but for most proteins a yield of 5 to 30 mg can be expected. The offer is only valid for proteins which are solubly expressed in HEK/CHO cells and which need no further optimization of the expression protocol. Additionally, the offer is only valid for the synthesis of standard genes with a GC content of 40-60%, no repeats, non-toxic for human, E. coli, HEK/CHO cells and which are not on the export control list.
For genes longer than 3 kbp and/or cloning into a custom expression vector, an extra fee applies. The time of delivery depends on the length of the ordered gene. The offer includes one purification step with affinity purification (his tag, protein A). A second purification (e.g. SEC, IEC) can be ordered separately.

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